High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system
Journal article
Narisetty, V., Amraoui, Y., Abdullah, A., Ahmad, E., Agrawal, D., Parameswaran, B., Pandey, A., Goel, S. and Kumar, V. (2021). High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system. Bioresource Technology. 337, p. 125463. https://doi.org/10.1016/j.biortech.2021.125463
Authors | Narisetty, V., Amraoui, Y., Abdullah, A., Ahmad, E., Agrawal, D., Parameswaran, B., Pandey, A., Goel, S. and Kumar, V. |
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Abstract | Downstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermentation broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 ± 4.6% and 45.5 ± 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/L BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45). |
Keywords | Xylose; Enterobacter ludwigii; 2,3-Butanediol; Aqueous two-phase system; Ammonium sulphate, Isopropanol |
Year | 2021 |
Journal | Bioresource Technology |
Journal citation | 337, p. 125463 |
Publisher | Elsevier |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biortech.2021.125463 |
Publication dates | |
01 Oct 2021 | |
Online | 26 Jun 2021 |
Publication process dates | |
Accepted | 23 Jun 2021 |
Deposited | 20 Jul 2021 |
Accepted author manuscript | License File Access Level Open |
https://openresearch.lsbu.ac.uk/item/8x092
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Accepted author manuscript
Vivek et al., 2021 BDO downstream process manuscript.docx | ||
License: CC BY-NC-ND 4.0 | ||
File access level: Open |
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