3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration

Journal article


Stachewicz, U, Qiao, T, Rawlinson, SCF, Almeida, FV, Li, W-Q, Cattell, M and Barber, AH (2015). 3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration. Acta Biomaterialia. 27, pp. 88-100.
AuthorsStachewicz, U, Qiao, T, Rawlinson, SCF, Almeida, FV, Li, W-Q, Cattell, M and Barber, AH
Abstract

The interaction between resident cells and electrospun nanofibers is critical in determining resultant osteoblast proliferation and activity in orthopedic tissue scaffolds. The use of techniques to evaluate cell-nanofiber interactions is critical in understanding scaffold function, with visualization promising unparalleled access to spatial information on such interactions. 3D tomography exploiting focused ion beam (FIB)-scanning electron microscopy (SEM) was used to examine electrospun nanofiber scaffolds to understand the features responsible for (osteoblast-like MC3T3-E1 and UMR106) cell behavior and resultant scaffold function. 3D imaging of cell-nanofiber interactions within a range of electrospun poly(d,l-lactide-co-glycolide acid) (PLGA) nanofiber scaffold architectures indicated a coherent interface between osteoblasts and nanofiber surfaces, promoting osteoblast filopodia formation for successful cell growth. Coherent cell-nanofiber interfaces were demonstrated throughout a randomly organized and aligned nanofiber network. Gene expression of UMR106 cells grown on PLGA fibers did not deviate significantly from those grown on plastic, suggesting maintenance of phenotype. However, considerably lower expression of Ibsp and Alpl on PLGA fibers might indicate that these cells are still in the proliferative phase compared with a more differentiated cell on plastic. This work demonstrates the synergy between designing electrospun tissue scaffolds and providing comprehensive evaluation through high resolution imaging of resultant 3-dimensional cell growth within the scaffold.

KeywordsElectrospinning; Cells; Osteoblasts; Tissue scaffold; FIB; Electron microscopy; Image analysis; Bioengineering; MD Multidisciplinary; Biomedical Engineering
Year2015
JournalActa Biomaterialia
Journal citation27, pp. 88-100
ISSN1742-7061
Digital Object Identifier (DOI)doi:10.1016/j.actbio.2015.09.003
Publication dates
Print01 Nov 2015
Publication process dates
Deposited21 Aug 2018
Accepted04 Sep 2015
Accepted author manuscript
License
CC BY-NC-ND 4.0
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https://openresearch.lsbu.ac.uk/item/875vz

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