3D nanomechanical evaluations of dermal structures in skin

Journal article


Kao, AP, Connelly, JT and Barber, AH (2015). 3D nanomechanical evaluations of dermal structures in skin. Journal of the Mechanical Behavior of Biomedical Materials. 57, pp. 14-23.
AuthorsKao, AP, Connelly, JT and Barber, AH
Abstract

Skin is a multilayered multiscale composite material with a range of mechanical and biochemical functions. The mechanical properties of dermis are important to understand in order to improve and compare on-going in vitro experiments to physiological conditions, especially as the mechanical properties of the dermis can play a crucial role in determining cell behaviour. Spatial and isotropy variations in dermal mechanics are thus critical in such understanding of complex skin structures. Atomic force microscopy (AFM) based indentation was used in this study to quantify the three dimensional mechanical properties of skin at nanoscale resolution over micrometre length scales. A range of preparation methods was examined and a mechanically non-evasive freeze sectioning
followed by thawing method was found to be suitable for the AFM studies. Subsequent mechanical evaluations established macroscale isotropy of the dermis with the ground
substance of the dermis dominating the mechanical response. Mechanical analysis was extended to show significant variation in the elastic modulus of the dermis between anatomical locations that suggest changes in the physiological environment influence local mechanical properties. Our results highlight dependence between an isotropic mechanical response of the dermal microenvironment at the nanoscale and anatomical
location that define the variable mechanical behaviour of the dermis.

Keywords0903 Biomedical Engineering; 0912 Materials Engineering; 0913 Mechanical Engineering; Biomedical Engineering
Year2015
JournalJournal of the Mechanical Behavior of Biomedical Materials
Journal citation57, pp. 14-23
PublisherElsevier
ISSN1751-6161
Digital Object Identifier (DOI)doi:10.1016/j.jmbbm.2015.11.017
Publication dates
Print28 Nov 2015
Publication process dates
Deposited21 Aug 2018
Accepted17 Nov 2015
Accepted author manuscript
License
CC BY-NC-ND 4.0
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https://openresearch.lsbu.ac.uk/item/8758w

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