Type VI Collagen Regulates Dermal Matrix Assembly and Fibroblast Motility

Journal article

Theocharidis, G, Drymoussi, Z, Kao, AP, Barber, AH, Lee, DA, Braun, KM and Connelly, JT (2016). Type VI Collagen Regulates Dermal Matrix Assembly and Fibroblast Motility. Journal of Investigative Dermatology. 136 (1), pp. 74-83. https://doi.org/10.1038/JID.2015.352
AuthorsTheocharidis, G, Drymoussi, Z, Kao, AP, Barber, AH, Lee, DA, Braun, KM and Connelly, JT
Abstract

Type VI collagen is a nonfibrillar collagen expressed in many connective tissues and implicated in extracellular matrix (ECM) organization. We hypothesized that type VI collagen regulates matrix assembly and cell function within the dermis of the skin. In the present study we examined the expression pattern of type VI collagen in normal and wounded skin and investigated its specific function in new matrix deposition by human dermal fibroblasts. Type VI collagen was expressed throughout the dermis of intact human skin, at the expanding margins of human keloid samples, and in the granulation tissue of newly deposited ECM in a mouse model of wound healing. Generation of cell-derived matrices (CDMs) by human dermal fibroblasts with stable knockdown of COL6A1 revealed that type VI collagen-deficient matrices were significantly thinner and contained more aligned, thicker, and widely spaced fibers than CDMs produced by normal fibroblasts. In addition, there was significantly less total collagen and sulfated proteoglycans present in the type VI collagen-depleted matrices. Normal fibroblasts cultured on de-cellularized CDMs lacking type VI collagen displayed increased cell spreading, migration speed, and persistence. Taken together, these findings indicate that type VI collagen is a key regulator of dermal matrix assembly, composition, and fibroblast behavior and may play an important role in wound healing and tissue regeneration.

Keywords1103 Clinical Sciences; 1112 Oncology And Carcinogenesis; Dermatology & Venereal Diseases
Year2016
JournalJournal of Investigative Dermatology
Journal citation136 (1), pp. 74-83
PublisherElsevier
ISSN0022-202X
Digital Object Identifier (DOI)https://doi.org/10.1038/JID.2015.352
Publication dates
Print04 Jan 2016
Publication process dates
Deposited22 Aug 2018
Accepted17 Aug 2015
Accepted author manuscript
License
File Access Level
Open
Permalink -

https://openresearch.lsbu.ac.uk/item/8754z

Download files

Accepted author manuscript
Type VI Collagen Regulates Dermal Matrix Assembly accepted version.pdf
License: CC BY 4.0
File access level: Open

  • 125
    total views
  • 167
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

Modal analysis of novel coronavirus (SARS COV-2) using finite element methodology
Warsame, C., Valerini, D., Llavori, I., Barber, A. and Goel, S. (2022). Modal analysis of novel coronavirus (SARS COV-2) using finite element methodology. Journal of the Mechanical Behavior of Biomedical Materials. 135, p. 105406. https://doi.org/10.1016/j.jmbbm.2022.105406
Biomimetic generation of the strongest known biomaterial found in limpet tooth.
Rumney, R., Robson, S., Kao, A., Barbu, E., Bozycki, L., Smith, J., Cragg, S., Couceiro, F., Parwani, R., Tozzi, G., Stuer, M., Barber, Asa H, Ford, A. and Górecki, D. (2022). Biomimetic generation of the strongest known biomaterial found in limpet tooth. Nature Communications. 13 (1), p. 3753. https://doi.org/10.1038/s41467-022-31139-0
Composite 3D printing of biomimetic human teeth
Barber, A. H., Cresswell-Boyes, A. J., Davies, G. R., Krishnamoorthy, M. and Mills, D. (2022). Composite 3D printing of biomimetic human teeth. Scientific Reports. 12 (7830), pp. 1-11. https://doi.org/10.1038/s41598-022-11658-y
X-ray computed tomography evaluations of additive manufactured multimaterial composites.
Curto, M., Kao, A P, Keeble, W, Tozzi, G. and Barber, A H (2021). X-ray computed tomography evaluations of additive manufactured multimaterial composites. Journal of Microscopy. https://doi.org/10.1111/jmi.13034
Resilient and Agile Engineering Solutions to Address Societal Challenges like Coronavirus Pandemic
Goel, S., Hawi, S., Goel, G., Thakur, V.K., Pearce, O., Hoskins, C., Hussain, T., Agrawal, A., Upadhyaya, H., Cross, G. and Barber, A. (2020). Resilient and Agile Engineering Solutions to Address Societal Challenges like Coronavirus Pandemic. Materials Today Chemistry. https://doi.org/10.1016/j.mtchem.2020.100300
Full-Field Strain Analysis of Bone–Biomaterial Systems Produced by the Implantation of Osteoregenerative Biomaterials in an Ovine Model
Peña Fernández, M., Dall’Ara, Enrico, Bodey, Andrew J., Parwani, Rachna, Barber, A., Blunn, Gordon W. and Tozzi, G. (2019). Full-Field Strain Analysis of Bone–Biomaterial Systems Produced by the Implantation of Osteoregenerative Biomaterials in an Ovine Model. ACS Biomaterials Science & Engineering. 5 (5), pp. 2543-2554. https://doi.org/10.1021/acsbiomaterials.8b01044
Seismo‐Mechanical Response of Anisotropic Rocks Under Hydraulic Fracture Conditions: New Experimental Insights
Gehne, S., Benson, P., Koor, N., Dobson, K., Enfield, M. and Barber, A. (2019). Seismo‐Mechanical Response of Anisotropic Rocks Under Hydraulic Fracture Conditions: New Experimental Insights. Journal of Geophysical Research: Solid Earth. 124 (9), pp. 9562-9579. https://doi.org/10.1029/2019jb017342
Structural orientation dependent sub-lamellar bone mechanics
Jimenez-Palomar, I, Shipov, A, Shahar, R and Barber, AH (2015). Structural orientation dependent sub-lamellar bone mechanics. Journal of the Mechanical Behavior of Biomedical Materials. 52, pp. 63-71. https://doi.org/10.1016/j.jmbbm.2015.02.031
Microscopy and supporting data for osteoblast integration within an electrospun fibrous network
Stachewicz, U, Qiao, T, Rawlinson, SCF, Veiga Almeida, F, Li, W-Q, Cattell, M and Barber, AH (2015). Microscopy and supporting data for osteoblast integration within an electrospun fibrous network. Data in Brief. 5, pp. 775-781. https://doi.org/10.1016/j.dib.2015.10.009
3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration
Stachewicz, U, Qiao, T, Rawlinson, SCF, Almeida, FV, Li, W-Q, Cattell, M and Barber, AH (2015). 3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration. Acta Biomaterialia. 27, pp. 88-100. https://doi.org/10.1016/j.actbio.2015.09.003
The cytolinker plectin regulates nuclear mechanotransduction in keratinocytes
Almeida, FV, Walko, G, McMillan, JR, McGrath, JA, Wiche, G, Barber, AH and Connelly, JT (2015). The cytolinker plectin regulates nuclear mechanotransduction in keratinocytes. Journal of Cell Science. 128 (24), pp. 4475-4486. https://doi.org/10.1242/jcs.173435
3D nanomechanical evaluations of dermal structures in skin
Kao, AP, Connelly, JT and Barber, AH (2015). 3D nanomechanical evaluations of dermal structures in skin. Journal of the Mechanical Behavior of Biomedical Materials. 57, pp. 14-23. https://doi.org/10.1016/j.jmbbm.2015.11.017
Nanointerfacial strength between non-collagenous protein and collagen fibrils in antler bone
Hang, F, Gupta, HS and Barber, AH (2013). Nanointerfacial strength between non-collagenous protein and collagen fibrils in antler bone. Journal of The Royal Society Interface. 11 (92), pp. 20130993-20130993. https://doi.org/10.1098/rsif.2013.0993
Adhesion Anisotropy between Contacting Electrospun Fibers
Stachewicz, U, Hang, F and Barber, AH (2014). Adhesion Anisotropy between Contacting Electrospun Fibers. Langmuir. 30 (23), pp. 6819-6825. https://doi.org/10.1021/la5004337
Polarised infrared microspectroscopy of edge-oriented graphene oxide papers
Frogley, MD, Wang, C, Cinque, G and Barber, AH (2014). Polarised infrared microspectroscopy of edge-oriented graphene oxide papers. Vibrational Spectroscopy. 75, pp. 178-183. https://doi.org/10.1016/j.vibspec.2014.07.005
Molecular force transfer mechanisms in graphene oxide paper evaluated using atomic force microscopy and in situ synchrotron micro FT-IR spectroscopy
Wang, C, Frogley, MD, Cinque, G, Liu, L-Q and Barber, AH (2014). Molecular force transfer mechanisms in graphene oxide paper evaluated using atomic force microscopy and in situ synchrotron micro FT-IR spectroscopy. Nanoscale. 6 (23), pp. 14404-14411. https://doi.org/10.1039/C4NR03646H
Mechanical Behavior of Osteoporotic Bone at Sub-Lamellar Length Scales
Jimenez-Palomar, I, Shipov, A, Shahar, R and Barber, AH (2015). Mechanical Behavior of Osteoporotic Bone at Sub-Lamellar Length Scales. Frontiers in Materials. 2. https://doi.org/10.3389/fmats.2015.00009
Extreme strength observed in limpet teeth
Barber, AH, Lu, D and Pugno, NM (2015). Extreme strength observed in limpet teeth. Journal of The Royal Society Interface. 12 (105), pp. 20141326-20141326. https://doi.org/10.1098/rsif.2014.1326
Wetting Hierarchy in Oleophobic 3D Electrospun Nanofiber Networks
Stachewicz, U, Bailey, RJ, Zhang, H, Stone, CA, Willis, CR and Barber, AH (2015). Wetting Hierarchy in Oleophobic 3D Electrospun Nanofiber Networks. ACS applied materials & interfaces. 7 (30), pp. 16645-16652. https://doi.org/10.1021/acsami.5b04272
Hydration dependent mechanical performance of denture adhesive hydrogels
Zhang, F, An, Y, Roohpour, N, Barber, AH and Gautrot, JE (2018). Hydration dependent mechanical performance of denture adhesive hydrogels. Dental Materials. 34 (10), pp. 1440-1448. https://doi.org/10.1016/j.dental.2018.06.015
Approaches to 3D printing teeth from X-ray microtomography.
Cresswell-Boyes, AJ, Barber, AH, Mills, D, Tatla, A and Davis, GR (2018). Approaches to 3D printing teeth from X-ray microtomography. Journal of Microscopy. 272 (3), pp. 207-212. https://doi.org/10.1111/jmi.12725
Optimization of digital volume correlation computation in SR-microCT images of trabecular bone and bone-biomaterial systems.
Peña Fernández, M, Barber, AH, Blunn, GW and Tozzi, G (2018). Optimization of digital volume correlation computation in SR-microCT images of trabecular bone and bone-biomaterial systems. Journal of Microscopy. 272 (3), pp. 213-272. https://doi.org/10.1111/jmi.12745
Development of sustainable biodegradable lignocellulosic hemp fiber/ polycaprolactone biocomposites for light weight applications
Barber, AH, Dhakal, HN, Ismail, S, Zhang, Z, Welsh, E, Maigret, J-E and Beaugrand, J (2018). Development of sustainable biodegradable lignocellulosic hemp fiber/ polycaprolactone biocomposites for light weight applications. Composites Part A: Applied Science and Manufacturing. 113, pp. 350-358. https://doi.org/10.1016/j.compositesa.2018.08.005
Preservation of bone tissue integrity with temperature control for in situ SR-MicroCT experiments
Fernández, MP, Dall'Ara, E, Kao, AP, Bodey, AJ, Karali, A, Blunn, GW, Barber, AH and Tozzi, G (2018). Preservation of bone tissue integrity with temperature control for in situ SR-MicroCT experiments. Materials. 11 (11). https://doi.org/10.3390/ma11112155
Effect of SR-microCT radiation on the mechanical integrity of trabecular bone using in situ mechanical testing and digital volume correlation
Peña Fernández, M, Cipiccia, S, Dall'Ara, E, Bodey, AJ, Parwani, R, Pani, M, Blunn, GW, Barber, AH and Tozzi, G (2018). Effect of SR-microCT radiation on the mechanical integrity of trabecular bone using in situ mechanical testing and digital volume correlation. Journal of the Mechanical Behavior of Biomedical Materials. 88, pp. 109-119. https://doi.org/10.1016/j.jmbbm.2018.08.012
Micro-mechanical properties of the tendon-to-bone attachment
Deymier, AC, An, Y, Boyle, JJ, Schwartz, AG, Birman, V, Genin, GM, Thomopoulos, S and Barber, AH (2017). Micro-mechanical properties of the tendon-to-bone attachment. Acta Biomaterialia. 56, pp. 25-35. https://doi.org/10.1016/j.actbio.2017.01.037
Surface free energy analysis of electrospun fibers based on Rayleigh-Plateau/Weber instabilities
Stachewicz, U, Dijksman, JF, Soudani, C, Tunnicliffe, LB, Busfield, JJC and Barber, AH (2017). Surface free energy analysis of electrospun fibers based on Rayleigh-Plateau/Weber instabilities. European Polymer Journal. 91, pp. 368-375. https://doi.org/10.1016/j.eurpolymj.2017.04.017
Extreme Toughness Exhibited in Electrospun Polystyrene Fibers
Zhang, F and Barber, AH (2017). Extreme Toughness Exhibited in Electrospun Polystyrene Fibers. Macromolecular Materials and Engineering. 302 (9), pp. 1700084-1700084. https://doi.org/10.1002/mame.201700084
X-ray Imaging of Transplanar Liquid Transport Mechanisms in Single Layer Textiles
Zhang, G, Parwani, R, Stone, CA, Barber, AH and Botto, L (2017). X-ray Imaging of Transplanar Liquid Transport Mechanisms in Single Layer Textiles. Langmuir. 33 (43), pp. 12072-12079. https://doi.org/10.1021/acs.langmuir.7b02982
Stress concentrations in nanoscale defective graphene
Wang, C, Wang, J and Barber, AH (2017). Stress concentrations in nanoscale defective graphene. AIP Advances. 7 (11), pp. 115001-115001. https://doi.org/10.1063/1.4996387
Evaluation of bioprosthetic heart valve failure using a matrix-fibril shear stress transfer approach
Anssari-Benam, A, Barber, AH and Bucchi, A (2016). Evaluation of bioprosthetic heart valve failure using a matrix-fibril shear stress transfer approach. Journal of Materials Science: Materials in Medicine. 27 (2). https://doi.org/10.1007/s10856-015-5657-2
Failure mechanisms in denture adhesives
An, Y, Li, D, Roohpour, N, Gautrot, JE and Barber, AH (2016). Failure mechanisms in denture adhesives. Dental Materials. 32 (5), pp. 615-623. https://doi.org/10.1016/j.dental.2016.01.007
Morphological and Mechanical Biomimetic Bone Structures
Parwani, R, Curto, M, Kao, AP, Rowley, PJ, Pani, M, Tozzi, G and Barber, AH (2016). Morphological and Mechanical Biomimetic Bone Structures. ACS Biomaterials Science & Engineering. 3 (11), pp. 2761-2767. https://doi.org/10.1021/acsbiomaterials.6b00652