The cytolinker plectin regulates nuclear mechanotransduction in keratinocytes

Journal article


Almeida, FV, Walko, G, McMillan, JR, McGrath, JA, Wiche, G, Barber, AH and Connelly, JT (2015). The cytolinker plectin regulates nuclear mechanotransduction in keratinocytes. Journal of Cell Science. 128 (24), pp. 4475-4486. https://doi.org/10.1242/jcs.173435
AuthorsAlmeida, FV, Walko, G, McMillan, JR, McGrath, JA, Wiche, G, Barber, AH and Connelly, JT
Abstract

The transmission of mechanical forces to the nucleus is important for intracellular positioning, mitosis and cell motility, yet the contribution of specific components of the cytoskeleton to nuclear mechanotransduction remains unclear. In this study, we examine how crosstalk between the cytolinker plectin and F-actin controls keratin network organisation and the 3D nuclear morphology of keratinocytes. Using micro-patterned surfaces to precisely manipulate cell shape, we find that cell adhesion and spreading regulate the size and shape of the nucleus. Disruption of the keratin cytoskeleton through loss of plectin facilitated greater nuclear deformation, which depended on acto-myosin contractility. Nuclear morphology did not depend on direct linkage of the keratin cytoskeleton with the nuclear membrane, rather loss of plectin reduced keratin filament density around the nucleus. We further demonstrate that keratinocytes have abnormal nuclear morphologies in the epidermis of plectin-deficient, epidermolysis bullosa simplex patients. Taken together, our data demonstrate that plectin is an essential regulator of nuclear morphology in vitro and in vivo and protects the nucleus from mechanical deformation.

KeywordsSkin; Mechanotransduction; Bioengineering; Imaging; Cell mechanics; 06 Biological Sciences; 11 Medical And Health Sciences; Developmental Biology
Year2015
JournalJournal of Cell Science
Journal citation128 (24), pp. 4475-4486
ISSN0021-9533
Digital Object Identifier (DOI)https://doi.org/10.1242/jcs.173435
Publication dates
Print02 Nov 2015
Publication process dates
Deposited21 Aug 2018
Accepted26 Oct 2015
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