Artificial Fusion Protein to Facilitate Calcium Carbonate Mineralization on Insoluble Polysaccharide for Efficient Biocementation

Journal article


Nawarathna, T.H.K, Nakashima, K., Kawabe, T., Mwandira, W., Kurumisawa, k. and Kawasaki, S. (2022). Artificial Fusion Protein to Facilitate Calcium Carbonate Mineralization on Insoluble Polysaccharide for Efficient Biocementation. ACS Sustainable Chemistry & Engineering. 9 (34), p. 11493–11502. https://doi.org/10.1021/acssuschemeng.1c03730
AuthorsNawarathna, T.H.K, Nakashima, K., Kawabe, T., Mwandira, W., Kurumisawa, k. and Kawasaki, S.
Abstract

Biomineralization is a process of mineral formation in living organisms. Compared with nonbiogenic minerals, biominerals can be defined as organic–inorganic hybrid materials that have excellent physical and optical properties. In the current study, an artificial protein mimicking the outer shell of crayfish, composed of CaCO3, chitin, and proteins, was developed to facilitate organic–inorganic hybrid material formation by precipitation of calcium carbonate on the chitin matrix. The fusion protein (CaBP-ChBD) was constructed by introducing a short-sequence calcite-binding peptide (CaBP) into the chitin-binding domain (ChBD). Calcium carbonate precipitation experiments by enzymatic urea hydrolysis revealed that a significant increase in the CaCO3 formation was achieved by adding CaBP-ChBD. Also, CaCO3 was efficiently deposited on chitin particles decorated with CaBP-ChBD. Most interestingly, CaBP-ChBD would improve the performance in sand solidification more efficiently and sustainably in the process of biocementation technique. The developed recombinant protein could be used for the sustainable production of organic–inorganic green materials for engineering applications.

Keywordsrecombinant protein, hybrid materials, biopolymer, calcium carbonate, biocementation
Year2022
JournalACS Sustainable Chemistry & Engineering
Journal citation9 (34), p. 11493–11502
PublisherAmerican Chemical Society (ACS)
ISSN2168-0485
Digital Object Identifier (DOI)https://doi.org/10.1021/acssuschemeng.1c03730
Web address (URL)https://pubs.acs.org/doi/abs/10.1021/acssuschemeng.1c03730
Publication dates
Print20 Aug 2021
Publication process dates
Accepted12 Aug 2021
Deposited27 Apr 2022
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Open
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