Combination of arsenic trioxide and cisplatin synergistically inhibits both hexokinase activity and viability of Ehrlich ascites carcinoma cells

Journal article


Mansour, Mohammed A, Ibrahim, Wafaa M, Shalaan, Eman S and Salama, Afrah F (2019). Combination of arsenic trioxide and cisplatin synergistically inhibits both hexokinase activity and viability of Ehrlich ascites carcinoma cells. Journal of biochemical and molecular toxicology. 33, pp. e22350-e22350. https://doi.org/10.1002/jbt.22350
AuthorsMansour, Mohammed A, Ibrahim, Wafaa M, Shalaan, Eman S and Salama, Afrah F
Abstract

Hexokinase‐2 is overexpressed in several carcinomas including breast cancer to sustain energy for rapidly dividing cells and associates with chemoresistance. However, the impact of chemo drugs (alone or in combination) on hexokinase activity and autophagic cell death is unclear. In this report, we used an in vivo murine adenocarcinoma model to validate the effects of As2O3 and cisplatin on hexokinase activity and autophagic cancer cell death. We found that the two drugs inhibit hexokinase activity and induce autophagic marker, beclin 1 expression. Interestingly, combining As2O3 with cisplatin synergistically enhanced these effects and alleviated oxidative stress often encountered in As2O3 treatment. Altogether, our data provide direct evidence that inhibition of hexokinase activity and induction of autophagic cell death are mediating the antineoplastic effects of As2O3 and cisplatin. Our findings raise the potential of combining As2O3 with cisplatin as an approach to augment cisplatin‐induced cell death and combat cisplatin chemoresistance in cancer.

Year2019
JournalJournal of biochemical and molecular toxicology
Journal citation33, pp. e22350-e22350
PublisherWiley
Digital Object Identifier (DOI)https://doi.org/10.1002/jbt.22350
Publication dates
Print31 May 2019
Publication process dates
Accepted07 May 2019
Deposited10 Aug 2020
Accepted author manuscript
License
File Access Level
Open
Additional information

This is the peer reviewed version of the following article: Combination of arsenic trioxide and cisplatin synergistically inhibits both hexokinase activity and viability of Ehrlich ascites carcinoma cells, which has been published in final form at https://doi.org/10.1002/jbt.22350. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Version

Permalink -

https://openresearch.lsbu.ac.uk/item/8q3qx

Download files


Accepted author manuscript
Text (inc Table and Figures) MANSOUR et al.docx
License: CC BY-NC 4.0
File access level: Open

  • 11
    total views
  • 11
    total downloads
  • 2
    views this month
  • 3
    downloads this month

Export as

Related outputs

Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells.
El-Gowily, A.H., Loutfy, S.A., Ali, E., Mohamed, T. and Mansour, M. (2021). Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells. Pharmaceuticals. 14 (3). https://doi.org/ph14030254
SP3 is associated with migration, invasion, and Akt/PKB signalling in MDA‐MB‐231 breast cancer cells
Mansour, M. (2020). SP3 is associated with migration, invasion, and Akt/PKB signalling in MDA‐MB‐231 breast cancer cells. Journal of biochemical and molecular toxicology. 35 (3). https://doi.org/10.1002/jbt.22657
Dual inhibition of glycolysis and autophagy as a therapeutic strategy in the treatment of Ehrlich ascites carcinoma
Mansour, MA, Ibrahim, WM, Salama, MM and Salama, AF (2020). Dual inhibition of glycolysis and autophagy as a therapeutic strategy in the treatment of Ehrlich ascites carcinoma. Journal of Biochemical and Molecular Toxicology. 34 (7). https://doi.org/10.1002/jbt.22498
Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model.
Mansour, M.A., Salama, A.F., Ibrahim, W.M. and Shalaan, E.S. (2019). Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. Alexandria Journal for Veterinary Sciences. 61 (1), pp. 159-167. https://doi.org/10.5455/ajvs.29544
Cisplatin augments the anti-schistosomal effect of praziquantel in a schistosoma-infected cancer model
Salem, M.L., Salama, A., El-Gowily, A.H., Mansour, M.A. and El-Said, M.M.A. (2019). Cisplatin augments the anti-schistosomal effect of praziquantel in a schistosoma-infected cancer model. Indian Journal of Biochemistry and Biophysics (IJBB). 56, pp. 57-69.
Pd (II) and Pt (II) chalcone complexes. Synthesis, spectral characterization, molecular modeling, biomolecular docking, antimicrobial and antitumor activities
Gaber, M., El-Ghamry, H.A and Mansour, M.A. (2018). Pd (II) and Pt (II) chalcone complexes. Synthesis, spectral characterization, molecular modeling, biomolecular docking, antimicrobial and antitumor activities. Journal of Photochemistry and Photobiology A: Chemistry. 354, pp. 163-174. https://doi.org/10.1016/j.jphotochem.2017.07.031
Nano-synthesis, characterization, modeling and molecular docking analysis of Mn (II), Co (II), Cr (III) and Cu (II) complexes with azo pyrazolone ligand as new favorable antimicrobial and antitumor agents
Gaber, M., Khedr, A.M., Mansour, M.A. and Elsharkawy, M. (2018). Nano-synthesis, characterization, modeling and molecular docking analysis of Mn (II), Co (II), Cr (III) and Cu (II) complexes with azo pyrazolone ligand as new favorable antimicrobial and antitumor agents. Applied Organometallic Chemistry. 32, pp. e4606-e4606. https://doi.org/10.1002/aoc.4606
Synthesis, spectroscopic, thermal and molecular modeling studies of Zn2+, Cd2+ and UO22+ complexes of Schiff bases containing triazole moiety. Antimicrobial, anticancer, antioxidant and DNA binding studies
Gaber, M., El-Ghamry, H.A., Fathalla, S.K. and Mansour, M.A. (2018). Synthesis, spectroscopic, thermal and molecular modeling studies of Zn2+, Cd2+ and UO22+ complexes of Schiff bases containing triazole moiety. Antimicrobial, anticancer, antioxidant and DNA binding studies. Materials Science and Engineering: C. 83, pp. 78-89. https://doi.org/10.1016/j.msec.2017.11.004
The phospholipid PI (3, 4) P 2 is an apical identity determinant
Román-Fernández, Á., Roignot, J., Sandilands, E., Nacke, M., Mansour, M.A., McGarry, L., Shanks, E., Mostov, K.E. and Bryant, D.M. (2018). The phospholipid PI (3, 4) P 2 is an apical identity determinant. Nature Communications. 9, pp. 1-17. https://doi.org/10.1038/s41467-018-07464-8
Ubiquitination: Friend and foe in cancer
Mansour, M.A. (2018). Ubiquitination: Friend and foe in cancer. The international journal of biochemistry & cell biology. 101, pp. 80-93. https://doi.org/10.1016/j.biocel.2018.06.001
FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression
Akter, K.A., Mansour, M.A., Hyodo, T. and Senga, T. (2017). FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression. The international journal of biochemistry & cell biology. 84, pp. 1-13. https://doi.org/10.1016/j.biocel.2016.12.013
HOXD8 exerts a tumor-suppressing role in colorectal cancer as an apoptotic inducer
Mansour, M.A. and Senga, T. (2017). HOXD8 exerts a tumor-suppressing role in colorectal cancer as an apoptotic inducer. The international journal of biochemistry & cell biology. 88, pp. 1-13. https://doi.org/10.1016/j.biocel.2017.04.011
SATB1 and SATB2 play opposing roles in c-Myc expression and progression of colorectal cancer
Mansour, MA, Hyodo, T, Akter, KA, Kokuryo, T, Uehara, K, Nagino, M and Senga, T (2016). SATB1 and SATB2 play opposing roles in c-Myc expression and progression of colorectal cancer. Oncotarget. 7, pp. 4993-4993. https://doi.org/10.18632/oncotarget.6651
UBE2S is associated with malignant characteristics of breast cancer cells
Ayesha, A K, Hyodo, T, Asano, E, Sato, N, Mansour, M A, Ito, S, Hamaguchi, M and Senga, T (2016). UBE2S is associated with malignant characteristics of breast cancer cells. Tumor Biology. 37 (1), pp. 763-772. https://doi.org/10.1007/s13277-015-3863-7
FAM98A is a novel substrate of PRMT1 required for tumor cell migration, invasion, and colony formation
Akter, K.A., Mansour, M.A., Hyodo, T., Ito, S., Hamaguchi, M. and Senga, T. (2016). FAM98A is a novel substrate of PRMT1 required for tumor cell migration, invasion, and colony formation. Tumor Biology. 37, pp. 4531-4539. https://doi.org/10.1007/s13277-015-4310-5
TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion
Kurita, K, Maeda, M, Mansour, MA, Kokuryo, T, Uehara, K, Yokoyama, Y, Nagino, M, Hamaguchi, M and Senga, T (2016). TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion. Oncology letters. 12, pp. 5240-5246. https://doi.org/10.3892/ol.2016.5332
Special AT-rich sequence-binding protein 2 suppresses invadopodia formation in HCT116 cells via palladin inhibition
Mansour, M.A., Asano, E., Hyodo, T., Akter, K.A., Takahashi, M., Hamaguchi, M. and Senga, T. (2015). Special AT-rich sequence-binding protein 2 suppresses invadopodia formation in HCT116 cells via palladin inhibition. Experimental Cell Research. 332, pp. 78-88. https://doi.org/10.1016/j.yexcr.2014.12.003
SATB 2 suppresses the progression of colorectal cancer cells via inactivation of MEK 5/ERK 5 signaling
Mansour, M.A., Hyodo, T., Ito, S., Kurita, K., Kokuryo, T., Uehara, K., Nagino, M., Takahashi, M., Hamaguchi, M. and Senga, T. (2015). SATB 2 suppresses the progression of colorectal cancer cells via inactivation of MEK 5/ERK 5 signaling. The FEBS journal. 282 (8), pp. 1394-1405. https://doi.org/10.1111/febs.13227
Treatment with folic acid ameliorated the histopathological alterations caused by propylthiouracil-induced hypothyroid rat testes
Tousson, E., Ali, E.M.M., Ibrahim, W. and Mansour, M.A. (2012). Treatment with folic acid ameliorated the histopathological alterations caused by propylthiouracil-induced hypothyroid rat testes. Toxicology and industrial health. 28 (6), pp. 566-576. https://doi.org/10.1177/0748233711420469
Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats
Ibrahim, W., Tousson, E., Ali, E.M.M. and Mansour, M.A. (2011). Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats. General and comparative endocrinology. 174, pp. 143-149. https://doi.org/10.1016/j.ygcen.2011.08.012
Proliferating cell nuclear antigen as a molecular biomarker for spermatogenesis in PTU-induced hypothyroidism of rats
Tousson, E., Ali, E.M.M., Ibrahim, W. and Mansour, M.A. (2011). Proliferating cell nuclear antigen as a molecular biomarker for spermatogenesis in PTU-induced hypothyroidism of rats. Reproductive sciences. 18, pp. 679-686. https://doi.org/10.1177/1933719110395401