Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model.

Journal article


Mansour, M.A., Salama, A.F., Ibrahim, W.M. and Shalaan, E.S. (2019). Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. Alexandria Journal for Veterinary Sciences. 61 (1), pp. 159-167. https://doi.org/10.5455/ajvs.29544
AuthorsMansour, M.A., Salama, A.F., Ibrahim, W.M. and Shalaan, E.S.
Abstract

Arsenic trioxide (As2O3) (ATO) and cisplatin (CIS) have potent antineoplastic effects in several types of cancer. Autophagy is important for normal cell function and survival, it is also used by tumor cells so, we studied it's role as possible mechanism of ATO and/or CIS antitumor effect on mice bearing Ehrlich ascites carcinoma (EAC) and checked whether ATO can enhance the antitumor potential of CIS. The study was carried out on eight groups of female mice; GP1 (negative control), GP2 (Erlich tumor only), GP3 (Normal +ATO), GP4 (Normal + CIS), GP5 (Normal + ATO+CIS), GP6 (EAC+ATO), GP7 (EAC+CIS) and GP8 (EAC +ATO+ CIS). In this study, viable cells were counted, percentage of viability (%) was calculated. Flowcytometry of autophagosome was appointed. Glutathione S-transferase (GST) and catalase (CAT) enzymes activities, total thiol and malondialdehyde (MDA) concentrations in liver tissues were determined to evaluate the effects of these drugs. Treatment with ATO (GP6) induced a significant decrease in tumor volume and percentage of viability with best result in combination of ATO with low dose of CIS (GP8) against EAC. Our results showed that a reduction in flourscence intensity of autophagosome marker that determined by flowcytometry especially in combination treated group (GP8). CAT, GST enzymes activities and total thiol level were decreased, while MDA level was increased in ATO treated group (GP6) and CIS treated group (GP7) as compared to EAC group. On other hand, combining both ATO and CIS in EAC treated group (GP8) decreased MDA level and augmented the level of total thiol and activities of CAT and GST enzymes. Therefore, our result revealed that combination of ATO and CIS have anti-tumor effects against EAC better than each one alone. So, we recommended the combination of ATO with CIS treatment due to their synergetic effect on cancer

Year2019
JournalAlexandria Journal for Veterinary Sciences
Journal citation61 (1), pp. 159-167
Digital Object Identifier (DOI)https://doi.org/10.5455/ajvs.29544
Publication dates
Print2019
Publication process dates
Deposited07 Aug 2020
Publisher's version
License
File Access Level
Open
Permalink -

https://openresearch.lsbu.ac.uk/item/8q3qz

Download files


Publisher's version
AlexJournal of VetSci_31-1549240255.pdf
License: CC BY-NC-SA 4.0
File access level: Open

  • 17
    total views
  • 18
    total downloads
  • 2
    views this month
  • 3
    downloads this month

Export as

Related outputs

Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells.
El-Gowily, A.H., Loutfy, S.A., Ali, E., Mohamed, T. and Mansour, M. (2021). Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells. Pharmaceuticals. 14 (3). https://doi.org/ph14030254
SP3 is associated with migration, invasion, and Akt/PKB signalling in MDA‐MB‐231 breast cancer cells
Mansour, M. (2020). SP3 is associated with migration, invasion, and Akt/PKB signalling in MDA‐MB‐231 breast cancer cells. Journal of biochemical and molecular toxicology. 35 (3). https://doi.org/10.1002/jbt.22657
Dual inhibition of glycolysis and autophagy as a therapeutic strategy in the treatment of Ehrlich ascites carcinoma
Mansour, MA, Ibrahim, WM, Salama, MM and Salama, AF (2020). Dual inhibition of glycolysis and autophagy as a therapeutic strategy in the treatment of Ehrlich ascites carcinoma. Journal of Biochemical and Molecular Toxicology. 34 (7). https://doi.org/10.1002/jbt.22498
Combination of arsenic trioxide and cisplatin synergistically inhibits both hexokinase activity and viability of Ehrlich ascites carcinoma cells
Mansour, Mohammed A, Ibrahim, Wafaa M, Shalaan, Eman S and Salama, Afrah F (2019). Combination of arsenic trioxide and cisplatin synergistically inhibits both hexokinase activity and viability of Ehrlich ascites carcinoma cells. Journal of biochemical and molecular toxicology. 33, pp. e22350-e22350. https://doi.org/10.1002/jbt.22350
Cisplatin augments the anti-schistosomal effect of praziquantel in a schistosoma-infected cancer model
Salem, M.L., Salama, A., El-Gowily, A.H., Mansour, M.A. and El-Said, M.M.A. (2019). Cisplatin augments the anti-schistosomal effect of praziquantel in a schistosoma-infected cancer model. Indian Journal of Biochemistry and Biophysics (IJBB). 56, pp. 57-69.
Pd (II) and Pt (II) chalcone complexes. Synthesis, spectral characterization, molecular modeling, biomolecular docking, antimicrobial and antitumor activities
Gaber, M., El-Ghamry, H.A and Mansour, M.A. (2018). Pd (II) and Pt (II) chalcone complexes. Synthesis, spectral characterization, molecular modeling, biomolecular docking, antimicrobial and antitumor activities. Journal of Photochemistry and Photobiology A: Chemistry. 354, pp. 163-174. https://doi.org/10.1016/j.jphotochem.2017.07.031
Nano-synthesis, characterization, modeling and molecular docking analysis of Mn (II), Co (II), Cr (III) and Cu (II) complexes with azo pyrazolone ligand as new favorable antimicrobial and antitumor agents
Gaber, M., Khedr, A.M., Mansour, M.A. and Elsharkawy, M. (2018). Nano-synthesis, characterization, modeling and molecular docking analysis of Mn (II), Co (II), Cr (III) and Cu (II) complexes with azo pyrazolone ligand as new favorable antimicrobial and antitumor agents. Applied Organometallic Chemistry. 32, pp. e4606-e4606. https://doi.org/10.1002/aoc.4606
Synthesis, spectroscopic, thermal and molecular modeling studies of Zn2+, Cd2+ and UO22+ complexes of Schiff bases containing triazole moiety. Antimicrobial, anticancer, antioxidant and DNA binding studies
Gaber, M., El-Ghamry, H.A., Fathalla, S.K. and Mansour, M.A. (2018). Synthesis, spectroscopic, thermal and molecular modeling studies of Zn2+, Cd2+ and UO22+ complexes of Schiff bases containing triazole moiety. Antimicrobial, anticancer, antioxidant and DNA binding studies. Materials Science and Engineering: C. 83, pp. 78-89. https://doi.org/10.1016/j.msec.2017.11.004
The phospholipid PI (3, 4) P 2 is an apical identity determinant
Román-Fernández, Á., Roignot, J., Sandilands, E., Nacke, M., Mansour, M.A., McGarry, L., Shanks, E., Mostov, K.E. and Bryant, D.M. (2018). The phospholipid PI (3, 4) P 2 is an apical identity determinant. Nature Communications. 9, pp. 1-17. https://doi.org/10.1038/s41467-018-07464-8
Ubiquitination: Friend and foe in cancer
Mansour, M.A. (2018). Ubiquitination: Friend and foe in cancer. The international journal of biochemistry & cell biology. 101, pp. 80-93. https://doi.org/10.1016/j.biocel.2018.06.001
FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression
Akter, K.A., Mansour, M.A., Hyodo, T. and Senga, T. (2017). FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression. The international journal of biochemistry & cell biology. 84, pp. 1-13. https://doi.org/10.1016/j.biocel.2016.12.013
HOXD8 exerts a tumor-suppressing role in colorectal cancer as an apoptotic inducer
Mansour, M.A. and Senga, T. (2017). HOXD8 exerts a tumor-suppressing role in colorectal cancer as an apoptotic inducer. The international journal of biochemistry & cell biology. 88, pp. 1-13. https://doi.org/10.1016/j.biocel.2017.04.011
SATB1 and SATB2 play opposing roles in c-Myc expression and progression of colorectal cancer
Mansour, MA, Hyodo, T, Akter, KA, Kokuryo, T, Uehara, K, Nagino, M and Senga, T (2016). SATB1 and SATB2 play opposing roles in c-Myc expression and progression of colorectal cancer. Oncotarget. 7, pp. 4993-4993. https://doi.org/10.18632/oncotarget.6651
UBE2S is associated with malignant characteristics of breast cancer cells
Ayesha, A K, Hyodo, T, Asano, E, Sato, N, Mansour, M A, Ito, S, Hamaguchi, M and Senga, T (2016). UBE2S is associated with malignant characteristics of breast cancer cells. Tumor Biology. 37 (1), pp. 763-772. https://doi.org/10.1007/s13277-015-3863-7
FAM98A is a novel substrate of PRMT1 required for tumor cell migration, invasion, and colony formation
Akter, K.A., Mansour, M.A., Hyodo, T., Ito, S., Hamaguchi, M. and Senga, T. (2016). FAM98A is a novel substrate of PRMT1 required for tumor cell migration, invasion, and colony formation. Tumor Biology. 37, pp. 4531-4539. https://doi.org/10.1007/s13277-015-4310-5
TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion
Kurita, K, Maeda, M, Mansour, MA, Kokuryo, T, Uehara, K, Yokoyama, Y, Nagino, M, Hamaguchi, M and Senga, T (2016). TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion. Oncology letters. 12, pp. 5240-5246. https://doi.org/10.3892/ol.2016.5332
Special AT-rich sequence-binding protein 2 suppresses invadopodia formation in HCT116 cells via palladin inhibition
Mansour, M.A., Asano, E., Hyodo, T., Akter, K.A., Takahashi, M., Hamaguchi, M. and Senga, T. (2015). Special AT-rich sequence-binding protein 2 suppresses invadopodia formation in HCT116 cells via palladin inhibition. Experimental Cell Research. 332, pp. 78-88. https://doi.org/10.1016/j.yexcr.2014.12.003
SATB 2 suppresses the progression of colorectal cancer cells via inactivation of MEK 5/ERK 5 signaling
Mansour, M.A., Hyodo, T., Ito, S., Kurita, K., Kokuryo, T., Uehara, K., Nagino, M., Takahashi, M., Hamaguchi, M. and Senga, T. (2015). SATB 2 suppresses the progression of colorectal cancer cells via inactivation of MEK 5/ERK 5 signaling. The FEBS journal. 282 (8), pp. 1394-1405. https://doi.org/10.1111/febs.13227
Treatment with folic acid ameliorated the histopathological alterations caused by propylthiouracil-induced hypothyroid rat testes
Tousson, E., Ali, E.M.M., Ibrahim, W. and Mansour, M.A. (2012). Treatment with folic acid ameliorated the histopathological alterations caused by propylthiouracil-induced hypothyroid rat testes. Toxicology and industrial health. 28 (6), pp. 566-576. https://doi.org/10.1177/0748233711420469
Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats
Ibrahim, W., Tousson, E., Ali, E.M.M. and Mansour, M.A. (2011). Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats. General and comparative endocrinology. 174, pp. 143-149. https://doi.org/10.1016/j.ygcen.2011.08.012
Proliferating cell nuclear antigen as a molecular biomarker for spermatogenesis in PTU-induced hypothyroidism of rats
Tousson, E., Ali, E.M.M., Ibrahim, W. and Mansour, M.A. (2011). Proliferating cell nuclear antigen as a molecular biomarker for spermatogenesis in PTU-induced hypothyroidism of rats. Reproductive sciences. 18, pp. 679-686. https://doi.org/10.1177/1933719110395401