The effect of amidation on the behaviour of antimicrobial peptides
Journal article
Mura, M, Wang, J, Zhou, Y, Pinna, M, Zvelindovsky, A, Dennison, SR and Phoenix, DA (2016). The effect of amidation on the behaviour of antimicrobial peptides. European Biophysics Journal. 45 (3), pp. 195-207. https://doi.org/10.1007/s00249-015-1094-x
Authors | Mura, M, Wang, J, Zhou, Y, Pinna, M, Zvelindovsky, A, Dennison, SR and Phoenix, DA |
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Abstract | Aurein 2.6-COOH and aurein 3.1-COOH were studied along with their naturally occurring C-terminally amidated analogues. Circular dichroism (CD) and molecular dynamic (MD) simulations were used to study the effects of amidation on the interaction of antimicrobial peptides (AMPs) with lipid bilayers. CD measurements and MD analysis suggested that both peptide analogues were predominantly random coil and adopted low levels of (Formula presented.)-helical structure in solution (<30 %) and in the presence of a lipid bilayer the peptides formed a stable (Formula presented.)-helical structure. In general, amidated analogues have a greater propensity than the non-amidated peptides to form a (Formula presented.)-helical structure. MD simulations predicted that aurein 2.6-COOH and aurein 3.1-CHOOH destabilised lipid bilayers from 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-phosphoserine via angled bilayer penetration. They also showed that aurein 2.6-CONH(Formula presented.) and aurein 3.1-CONH(Formula presented.) formed a helix horizontal to the plane of an asymmetric interface. © 2016 The Author(s) |
Keywords | Amino acids; Amino acid; Anticancer; Antimicrobial peptides; Cooperative effect; Membrane; Molecular dynamics; Secondary structure |
Year | 2016 |
Journal | European Biophysics Journal |
Journal citation | 45 (3), pp. 195-207 |
Publisher | Springer |
ISSN | 0175-7571 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00249-015-1094-x |
Funder/Client | Volkswagen Foundation |
Publication dates | |
08 Jan 2016 | |
Publication process dates | |
Deposited | 14 Apr 2016 |
Accepted | 20 Oct 2015 |
Publisher's version | License File description Article File Access Level Open |
Supplemental file | License File description Supplementary data |
License | http://creativecommons.org/licenses/by/4.0 |
https://openresearch.lsbu.ac.uk/item/87544
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Publisher's version
art_10.1007_s00249-015-1094-x1.pdf | ||
License: CC BY 4.0 | ||
File access level: Open |
Supplemental file
249_2015_1094_MOESM1_ESM.pdf | ||
License: CC BY 4.0 |
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