Investigations into the potential anticancer activity of Maximin H5
Journal article
Dennison, SR, Harris, F and Phoenix, DA (2017). Investigations into the potential anticancer activity of Maximin H5. Biochimie. 137 (June), pp. 29-34. https://doi.org/10.1016/j.biochi.2017.02.013
Authors | Dennison, SR, Harris, F and Phoenix, DA |
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Abstract | Here we report the first major example of anionic amphibian host defence peptides (HDPs) with anticancer activity. Maximin H5 is a C-terminally amidated, anionic host defence peptide (MH5N) from toads of the Bombina genus, which was shown to possess activity against the glioma cell line, T98G (EC50 = 125 μM). The peptide adopted high levels of α-helical structure (57.3%) in the presence of model cancer membranes (DMPC:DMPS in a molar ratio of 10:1). MH5N also showed a strong ability to penetrate these model membranes (Π = 10.5 mN m-1), which correlated with levels of DMPS (R2 > 0.98). Taken with the high ability of the peptide to lyse these membranes (65.7%), it is proposed that maximin H5 kills cancer cells via membranolytic mechanisms that are promoted by anionic lipid. It was also found that C-terminally deaminated maximin H5 (MH5C) exhibited lower levels of α-helical structure in the presence of cancer membrane mimics (44.8%) along with a reduced ability to penetrate these membranes (Π = 8.1 mN m-1) and induce their lysis (56.6%). These data suggested that the two terminal amide groups of native maximin H5 are required for its optimal membranolytic and anticancer activity. |
Keywords | Biochemistry And Cell Biology; Biochemistry & Molecular Biology |
Year | 2017 |
Journal | Biochimie |
Journal citation | 137 (June), pp. 29-34 |
Publisher | Elsevier BV |
ISSN | 0300-9084 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biochi.2017.02.013 |
Publication dates | |
27 Feb 2017 | |
Publication process dates | |
Deposited | 13 Mar 2017 |
Accepted | 24 Feb 2017 |
Accepted author manuscript | License File Access Level Open |
https://openresearch.lsbu.ac.uk/item/87053
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Accepted author manuscript
maxim 5 anticancer paper.docx | ||
License: CC BY-NC-ND 4.0 | ||
File access level: Open |
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