Erratum to: HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication

Journal article


Mehta, K, Farnaud, S and Patel, VB (2017). Erratum to: HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication. Molecular Biology Reports. 44 (5), pp. 405-405.
AuthorsMehta, K, Farnaud, S and Patel, VB
Abstract

Background: In liver hepatocytes, the HFE gene regulates cellular and systemic iron homeostasis by modulating cellular iron-uptake and producing the iron-hormone hepcidin in response to systemic iron elevation. However, the mechanism of iron-sensing in hepatocytes remain enigmatic. Therefore, to study the effect of iron on HFE and hepcidin (HAMP) expressions under distinct extracellular and intracellular iron-loading, we examined the effect of holotransferrin treatment (1,2, 5 and 8 g/L for 6 h) on intracellular iron levels, and mRNA expressions of HFE and HAMP in wild-type HepG2 and previously characterized iron-loaded recombinant-TfR1 HepG2 cells.

Methods and Results: Gene expression was analyzed by real-time PCR and intracellular iron was measured by ferrozine assay. Data showed that in the wild-type cells, where intracellular iron content remained unchanged, HFE expression remained unaltered at low holotransferrin treatments but was upregulated upon 5 g/L (p<0.04) and 8 g/L (p=0.05) treatments. HAMP expression showed alternating elevations and increased upon 1 g/L (p<0.05) and 5 g/L (p<0.05). However, in the recombinant cells that showed higher intracellular iron levels than wild-type cells, HFE and HAMP expressions were elevated only at low 1 g/L treatment (p<0.03) and were repressed at 2 g/L (p<0.03) treatment. Under holotransferrin-untreated conditions, the iron-loaded recombinant cells showed higher expressions of HFE (p<0.03) and HAMP (p=0.05) than wild-type cells.

Conclusions: HFE mRNA was independently elevated by extracellular and intracellular iron-excess. Thus, it may be involved in sensing both, extracellular and intracellular iron. Repression of HAMP expression under simultaneous intracellular and extracellular iron-loading resembles certain iron-excess pathologies.

Keywords0601 Biochemistry And Cell Biology; Biochemistry & Molecular Biology
Year2017
JournalMolecular Biology Reports
Journal citation44 (5), pp. 405-405
ISSN0301-4851
Digital Object Identifier (DOI)doi:10.1007/s11033-017-4127-y
Publication dates
Print19 Sep 2017
Publication process dates
Deposited02 Oct 2018
Accepted19 Sep 2017
Accepted author manuscript
License
CC BY 4.0
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