Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA

Book chapter


Mehta, K and Patel, V (2019). Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA. in: Hancock, John and Conway, Myra (ed.) Redox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990 Springer Science+Business Media, LLC, part of Springer Nature 2019.
AuthorsMehta, K and Patel, V
EditorsHancock, John and Conway, Myra
Abstract

Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.

Year2019
Book titleRedox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990
PublisherSpringer Science+Business Media, LLC, part of Springer Nature 2019
Edition1
Publication process dates
Deposited25 Apr 2019
Accepted02 Apr 2019
Accepted author manuscript
License
CC BY 4.0
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https://openresearch.lsbu.ac.uk/item/8670q

Accepted author manuscript

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