Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA
Mehta, K and Patel, V (2019). Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA. in: Hancock, John and Conway, Myra (ed.) Redox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990 Springer Science+Business Media, LLC, part of Springer Nature 2019.
|Authors||Mehta, K and Patel, V|
|Editors||Hancock, John and Conway, Myra|
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.
|Book title||Redox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990|
|Publisher||Springer Science+Business Media, LLC, part of Springer Nature 2019|
|Publication process dates|
|Deposited||25 Apr 2019|
|Accepted||02 Apr 2019|
|Accepted author manuscript|
CC BY 4.0
Accepted author manuscript
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