Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA

Book chapter

Mehta, K and Patel, V (2019). Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA. in: Hancock, John and Conway, Myra (ed.) Redox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990 Springer.
AuthorsMehta, K and Patel, V
EditorsHancock, John and Conway, Myra
Abstract

Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.

Year2019
Book titleRedox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990
PublisherSpringer
Edition1
Publication dates
Online31 May 2019
Publication process dates
Deposited25 Apr 2019
Accepted02 Apr 2019
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-4939-9463-2_4
Web address (URL)https://link.springer.com/protocol/10.1007/978-1-4939-9463-2_4
Accepted author manuscript
License
File Access Level
Open
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