Shotgun Metagenomic Sequencing Revealed the Prebiotic Potential of a Fruit Juice Drink with Fermentable Fibres in Healthy Humans

Journal article


Bester, A., O’Brien, M., Cotter, P.D., Dam, S. and Civai, C. (2023). Shotgun Metagenomic Sequencing Revealed the Prebiotic Potential of a Fruit Juice Drink with Fermentable Fibres in Healthy Humans. Foods. 12 (13), p. 2480. https://doi.org/10.3390/foods12132480
AuthorsBester, A., O’Brien, M., Cotter, P.D., Dam, S. and Civai, C.
Abstract

Fibre-based dietary interventions are at the forefront of gut microbiome modulation research, with a wealth of 16S rRNA information to demonstrate the prebiotic effects of isolated fibres. However, there is a distinct lack of data relating to the effect of a combination of soluble and insoluble fibres in a convenient-to-consume fruit juice food matrix on gut microbiota structure, diversity, and function. Here, we aimed to determine the impact of the MOJU Prebiotic Shot, an apple, lemon, ginger, and raspberry fruit juice drink blend containing chicory inulin, baobab, golden kiwi, and green banana powders, on gut microbiota structure and function. Healthy adults (n = 20) were included in a randomised, double-blind, placebo-controlled, cross-over study, receiving 60 mL MOJU Prebiotic Shot or placebo (without the fibre mix) for 3 weeks with a 3-week washout period between interventions. Shotgun metagenomics revealed significant between-group differences in alpha and beta diversity. In addition, the relative abundance of the phyla Actinobacteria and Desulfobacteria was significantly increased as a result of the prebiotic intervention. Nine species were observed to be differentially abundant (uncorrected p-value of <0.05) as a result of the prebiotic treatment. Of these, Bifidobacterium adolescentis and CAG-81 sp900066785 (Lachnospiraceae) were present at increased abundance relative to baseline. Additionally, KEGG analysis showed an increased abundance in pathways associated with arginine biosynthesis and phenylacetate degradation during the prebiotic treatment. Our results show the effects of the daily consumption of 60 mL MOJU Prebiotic Shot for 3 weeks and provide insight into the functional potential of B. adolescentis.

Year2023
JournalFoods
Journal citation12 (13), p. 2480
PublisherMDPI
ISSN2304-8158
Digital Object Identifier (DOI)https://doi.org/10.3390/foods12132480
Web address (URL)https://doi.org/10.3390/foods12132480
Publication dates
Online25 Jun 2023
Publication process dates
Accepted08 Jun 2023
Deposited27 Jun 2023
Publisher's version
License
File Access Level
Open
Permalink -

https://openresearch.lsbu.ac.uk/item/94519

Download files


Publisher's version
foods-12-02480-v2.pdf
License: CC BY 4.0
File access level: Open

  • 70
    total views
  • 71
    total downloads
  • 1
    views this month
  • 1
    downloads this month

Export as

Related outputs

Mental health of veterinary nurses and student veterinary nurses: A scoping review
King, N., Lewis, E., Kinnison, T., Langridge, A., Civai, C., May, S.A. and Cardwell, J.M. Mental health of veterinary nurses and student veterinary nurses: A scoping review. Veterinary Record. https://doi.org/10.1002/vetr.4091
Working memory and attention in choice.
Rustichini, A., Domenech, P., Civai, C. and DeYoung, Colin G (2023). Working memory and attention in choice. PLoS ONE. 18 (10), p. e0284127. https://doi.org/10.1371/journal.pone.0284127
How perceived scarcity predicted cooperation during early pandemic lockdown.
Civai, C., Caserotti, M., Carrus, E., Huijsmans, I. and Rubaltelli, E. (2022). How perceived scarcity predicted cooperation during early pandemic lockdown. Frontiers in Psychology. https://doi.org/10.3389/fpsyg.2022.951757
Diet and the microbiome in precision medicine
Toribio-Mateas, M. and Bester, A. (2021). Diet and the microbiome in precision medicine. in: Faintuch, Joel and Faintuch, Salomao (ed.) Precision Medicine for Investigators, Practitioners and Providers Academic Press. pp. 445-452
Impact of Plant-Based Meat Alternatives on the Gut Microbiota of Consumers: A Real-World Study
Toribio-Mateas, M., Bester, A. and Klimenko, N. (2021). Impact of Plant-Based Meat Alternatives on the Gut Microbiota of Consumers: A Real-World Study. Foods (Basel, Switzerland). 10 (9), p. 2040. https://doi.org/10.3390/foods10092040
Does unfairness sound wrong? A cross-domain investigation of expectations in music and social decision-making
Civai, C, Teodorini, R and Carrus, E (2020). Does unfairness sound wrong? A cross-domain investigation of expectations in music and social decision-making. Royal Society Open Science. 7 (9). https://doi.org/10.1098/rsos.190048
Mentalizing in value-based social decision-making: shaping expectations and social norms
Civai, C and Sanfey, A (2019). Mentalizing in value-based social decision-making: shaping expectations and social norms. in: Gilead, M and Ochsner, K (ed.) The Neural Basis of Mentalizing - A Social-Cognitive and Affective Neuroscience Perspective Springer.
A scarcity mindset alters neural processing underlying consumer decision making
Huijsmans, I, Ma, I, Micheli, L, Civai, C, Stallen, M and G Sanfey, A (2019). A scarcity mindset alters neural processing underlying consumer decision making. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1818572116
Neurocognitive mechanisms of reactions to second- and third-party justice violations.
Civai, C, Huijsmans, I and Sanfey, A (2019). Neurocognitive mechanisms of reactions to second- and third-party justice violations. Scientific Reports. 9. https://doi.org/10.1038/s41598-019-45725-8
Effects of serotonin depletion and dopamine depletion on bimodal divided attention.
Königschulte, W, Civai, C, Hildebrand, P, Gaber, TJ, Fink, GR and Zepf, FD (2018). Effects of serotonin depletion and dopamine depletion on bimodal divided attention. World Journal of Biological Psychiatry. https://doi.org/10.1080/15622975.2018.1532110
Game Theory in Neuroeconomics
Civai, C and Hawes, DR (2016). Game Theory in Neuroeconomics. in: Reuter, M and Montag, C (ed.) Neuroeconomics Springer. pp. 13-40
The Enhancement of Social Norm Compliance: Prospects and Caveats
Civai, C and Ma, I (2017). The Enhancement of Social Norm Compliance: Prospects and Caveats. Journal of Cognitive Enhancement. 1 (1), pp. 26-30. https://doi.org/10.1007/s41465-017-0009-4
Intelligence and Extraversion in the neural evaluation of delayed rewards
Civai, C, Hawes, DR, DeYoung, CG and Rustichini, A (2016). Intelligence and Extraversion in the neural evaluation of delayed rewards. Journal of Research in Personality. 61, pp. 99-108. https://doi.org/10.1016/j.jrp.2016.02.006
Predicting the other in cooperative interactions.
Sanfey, AG, Civai, C and Vavra, P (2015). Predicting the other in cooperative interactions. Trends in cognitive sciences. 19 (7), pp. 364-365. https://doi.org/10.1016/j.tics.2015.05.009
Medial prefrontal cortex reacts to unfairness if this damages the self: a tDCS study.
Civai, C, Miniussi, C and Rumiati, RI (2014). Medial prefrontal cortex reacts to unfairness if this damages the self: a tDCS study. Social Cognitive and Affective Neuroscience. 10 (8), pp. 1054-1060. https://doi.org/10.1093/scan/nsu154
Disentangling self- and fairness-related neural mechanisms involved in the ultimatum game: an fMRI study
Corradi-Dell'Acqua, C., Civai, C., Rumiati, R.I. and Fink, G.R. (2013). Disentangling self- and fairness-related neural mechanisms involved in the ultimatum game: an fMRI study. Social Cognitive and Affective Neuroscience. 8 (4), p. 424–431. https://doi.org/10.1093/scan/nss014
Rejecting unfairness: emotion-driven reaction or cognitive heuristic?
Civai, C (2013). Rejecting unfairness: emotion-driven reaction or cognitive heuristic? Frontiers in Human Neuroscience. 7, p. 126. https://doi.org/10.3389/fnhum.2013.00126
Framing the ultimatum game: the contribution of simulation.
Tomasino, B, Lotto, L, Sarlo, M, Civai, C, Rumiati, R and Rumiati, RI (2013). Framing the ultimatum game: the contribution of simulation. Frontiers in Human Neuroscience. 7, p. 337. https://doi.org/10.3389/fnhum.2013.00337