The Genomic Landscape of Actinic Keratosis

Journal article


Thomson, J., Bewicke-Copley, F., Anene, C., Gulati, A., Nagano, A., Purdie, K., Inman, G.J., Proby, C.M., Leigh, I.M., Harwood, C.A. and Wang, J. (2021). The Genomic Landscape of Actinic Keratosis. The Journal of Investigative Dermatology. https://doi.org/10.1016/j.jid.2020.12.024
AuthorsThomson, J., Bewicke-Copley, F., Anene, C., Gulati, A., Nagano, A., Purdie, K., Inman, G.J., Proby, C.M., Leigh, I.M., Harwood, C.A. and Wang, J.
Abstract

Actinic keratoses (AKs) are lesions of epidermal keratinocyte dysplasia and are precursors for invasive cutaneous squamous cell carcinoma (cSCC). Identifying the specific genomic alterations driving the progression from normal skin to skin with AK to skin with invasive cSCC is challenging because of the massive UVR-induced mutational burden characteristic at all stages of this progression. In this study, we report the largest AK whole-exome sequencing study to date and perform a mutational signature and candidate driver gene analysis on these lesions. We demonstrate in 37 AKs from both immunosuppressed and immunocompetent patients that there are significant similarities between AKs and cSCC in terms of mutational burden, copy number alterations, mutational signatures, and patterns of driver gene mutations. We identify 44 significantly mutated AK driver genes and confirm that these genes are similarly altered in cSCC. We identify azathioprine mutational signature in all AKs from patients exposed to the drug, providing further evidence for its role in keratinocyte carcinogenesis. cSCCs differ from AKs in having higher levels of intrasample heterogeneity. Alterations in signaling pathways also differ, with immune-related signaling and TGFβ signaling significantly more mutated in cSCC. Integrating our findings with independent gene expression datasets confirms that dysregulated TGFβ signaling may represent an important event in AK‒cSCC progression.

Year2021
JournalThe Journal of Investigative Dermatology
PublisherElsevier
ISSN1523-1747
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jid.2020.12.024
Web address (URL)https://www.jidonline.org/article/S0022-202X(21)00014-2/fulltext
Publication dates
Print21 Jun 2021
Online19 Jan 2021
Publication process dates
Accepted16 Dec 2020
Deposited23 Jun 2022
Accepted author manuscript
License
File Access Level
Open
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