The Genomic Landscape of Actinic Keratosis
Journal article
Thomson, J., Bewicke-Copley, F., Anene, C., Gulati, A., Nagano, A., Purdie, K., Inman, G.J., Proby, C.M., Leigh, I.M., Harwood, C.A. and Wang, J. (2021). The Genomic Landscape of Actinic Keratosis. The Journal of Investigative Dermatology. https://doi.org/10.1016/j.jid.2020.12.024
Authors | Thomson, J., Bewicke-Copley, F., Anene, C., Gulati, A., Nagano, A., Purdie, K., Inman, G.J., Proby, C.M., Leigh, I.M., Harwood, C.A. and Wang, J. |
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Abstract | Actinic keratoses (AKs) are lesions of epidermal keratinocyte dysplasia and are precursors for invasive cutaneous squamous cell carcinoma (cSCC). Identifying the specific genomic alterations driving the progression from normal skin to skin with AK to skin with invasive cSCC is challenging because of the massive UVR-induced mutational burden characteristic at all stages of this progression. In this study, we report the largest AK whole-exome sequencing study to date and perform a mutational signature and candidate driver gene analysis on these lesions. We demonstrate in 37 AKs from both immunosuppressed and immunocompetent patients that there are significant similarities between AKs and cSCC in terms of mutational burden, copy number alterations, mutational signatures, and patterns of driver gene mutations. We identify 44 significantly mutated AK driver genes and confirm that these genes are similarly altered in cSCC. We identify azathioprine mutational signature in all AKs from patients exposed to the drug, providing further evidence for its role in keratinocyte carcinogenesis. cSCCs differ from AKs in having higher levels of intrasample heterogeneity. Alterations in signaling pathways also differ, with immune-related signaling and TGFβ signaling significantly more mutated in cSCC. Integrating our findings with independent gene expression datasets confirms that dysregulated TGFβ signaling may represent an important event in AK‒cSCC progression. |
Year | 2021 |
Journal | The Journal of Investigative Dermatology |
Publisher | Elsevier |
ISSN | 1523-1747 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jid.2020.12.024 |
Web address (URL) | https://www.jidonline.org/article/S0022-202X(21)00014-2/fulltext |
Publication dates | |
21 Jun 2021 | |
Online | 19 Jan 2021 |
Publication process dates | |
Accepted | 16 Dec 2020 |
Deposited | 23 Jun 2022 |
Accepted author manuscript | License File Access Level Open |
https://openresearch.lsbu.ac.uk/item/8zyq2
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Accepted author manuscript
Thomson, J., Bewicke-Copley, F., Anene, C.A., Gulati, A., Nagano, A., Purdie, K., Inman, G.J., Proby, C.M., Leigh, I.M., Harwood, C.A. and Wang, J., 2021.pdf | ||
License: CC BY-NC-ND 4.0 | ||
File access level: Open |
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