SFPQ promotes an oncogenic transcriptomic state in melanoma

Journal article


Bi, O., Anene, C., Nsengimana, J., Roberts, W., Newton-Bishop, J. and Boyne, J.R. (2021). SFPQ promotes an oncogenic transcriptomic state in melanoma. Oncogene. 40, pp. 5192-5203. https://doi.org/10.1038/s41388-021-01912-4
AuthorsBi, O., Anene, C., Nsengimana, J., Roberts, W., Newton-Bishop, J. and Boyne, J.R.
Abstract

The multifunctional protein, splicing factor, proline- and glutamine-rich (SFPQ) has been implicated in numerous cancers often due to interaction with coding and non-coding RNAs, however, its role in melanoma remains unclear. We report that knockdown of SFPQ expression in melanoma cells decelerates several cancer-associated cell phenotypes, including cell growth, migration, epithelial to mesenchymal transition, apoptosis, and glycolysis. RIP-seq analysis revealed that the SFPQ-RNA interactome is reprogrammed in melanoma cells and specifically enriched with key melanoma-associated coding and long non-coding transcripts, including SOX10, AMIGO2 and LINC00511 and in most cases SFPQ is required for the efficient expression of these genes. Functional analysis of two SFPQ-enriched lncRNA, LINC00511 and LINC01234, demonstrated that these genes independently contribute to the melanoma phenotype and a more detailed analysis of LINC00511 indicated that this occurs in part via modulation of the miR-625-5p/PKM2 axis. Importantly, analysis of a large clinical cohort revealed that elevated expression of SFPQ in primary melanoma tumours may have utility as a prognostic biomarker. Together, these data suggest that SFPQ is an important driver of melanoma, likely due to SFPQ–RNA interactions promoting the expression of numerous oncogenic transcripts.

Year2021
JournalOncogene
Journal citation40, pp. 5192-5203
PublisherSpringer Nature
ISSN1476-5594
Digital Object Identifier (DOI)https://doi.org/10.1038/s41388-021-01912-4
Web address (URL)https://www.nature.com/articles/s41388-021-01912-4#citeas
Publication dates
Print03 Jul 2021
Publication process dates
Accepted17 Jun 2021
Deposited16 Jun 2022
Publisher's version
License
File Access Level
Open
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