SFPQ promotes an oncogenic transcriptomic state in melanoma
Journal article
Bi, O., Anene, C., Nsengimana, J., Roberts, W., Newton-Bishop, J. and Boyne, J.R. (2021). SFPQ promotes an oncogenic transcriptomic state in melanoma. Oncogene. 40, pp. 5192-5203. https://doi.org/10.1038/s41388-021-01912-4
Authors | Bi, O., Anene, C., Nsengimana, J., Roberts, W., Newton-Bishop, J. and Boyne, J.R. |
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Abstract | The multifunctional protein, splicing factor, proline- and glutamine-rich (SFPQ) has been implicated in numerous cancers often due to interaction with coding and non-coding RNAs, however, its role in melanoma remains unclear. We report that knockdown of SFPQ expression in melanoma cells decelerates several cancer-associated cell phenotypes, including cell growth, migration, epithelial to mesenchymal transition, apoptosis, and glycolysis. RIP-seq analysis revealed that the SFPQ-RNA interactome is reprogrammed in melanoma cells and specifically enriched with key melanoma-associated coding and long non-coding transcripts, including SOX10, AMIGO2 and LINC00511 and in most cases SFPQ is required for the efficient expression of these genes. Functional analysis of two SFPQ-enriched lncRNA, LINC00511 and LINC01234, demonstrated that these genes independently contribute to the melanoma phenotype and a more detailed analysis of LINC00511 indicated that this occurs in part via modulation of the miR-625-5p/PKM2 axis. Importantly, analysis of a large clinical cohort revealed that elevated expression of SFPQ in primary melanoma tumours may have utility as a prognostic biomarker. Together, these data suggest that SFPQ is an important driver of melanoma, likely due to SFPQ–RNA interactions promoting the expression of numerous oncogenic transcripts. |
Year | 2021 |
Journal | Oncogene |
Journal citation | 40, pp. 5192-5203 |
Publisher | Springer Nature |
ISSN | 1476-5594 |
Digital Object Identifier (DOI) | https://doi.org/10.1038/s41388-021-01912-4 |
Web address (URL) | https://www.nature.com/articles/s41388-021-01912-4#citeas |
Publication dates | |
03 Jul 2021 | |
Publication process dates | |
Accepted | 17 Jun 2021 |
Deposited | 16 Jun 2022 |
Publisher's version | License File Access Level Open |
https://openresearch.lsbu.ac.uk/item/8zyq0
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Publisher's version
Bi, O., Anene, C.A., Nsengimana, J., Shelton, M., Roberts, W., Newton-Bishop, J. and Boyne, J.R., 2021.pdf | ||
License: CC BY 4.0 | ||
File access level: Open |
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