Validation of a commercially available indirect assay for SARS-CoV-2 neutralising antibodies using a pseudotyped virus assay.

Journal article


Murray MJ, McIntosh M, Fullerton, C., Mahungu T, Wright E, Chatterton W, Gandy M and Reeves MB (2021). Validation of a commercially available indirect assay for SARS-CoV-2 neutralising antibodies using a pseudotyped virus assay. Journal of Infection. 82 (5), pp. 170-177. https://doi.org/10.1016/j.jinf.2021.03.010
AuthorsMurray MJ, McIntosh M, Fullerton, C., Mahungu T, Wright E, Chatterton W, Gandy M and Reeves MB
Abstract

Objectives
To assess whether a commercially available CE-IVD, ELISA-based surrogate neutralisation assay (cPass, Genscript) provides a genuine measure of SARS-CoV-2 neutralisation by human sera, and further to establish whether measuring responses against the RBD of S was a diagnostically useful proxy for responses against the whole S protein.

Methods
Serum samples from 30 patients were assayed for anti-NP responses, for ‘neutralisation’ by the surrogate neutralisation assay and for neutralisation by SARS-CoV-2 S pseudotyped virus assays utilising two target cell lines. Correlation between assays was measured using linear regression.

Results
The responses observed within the surrogate neutralisation assay demonstrated an extremely strong, highly significant positive correlation with those observed in both pseudotyped virus assays.

Conclusions
The tested ELISA-based surrogate assay provides an immunologically useful measure of functional immune responses in a much quicker and highly automatable fashion. It also reinforces that detection of anti-RBD neutralising antibodies alone is a powerful measure of the capacity to neutralise viral infection.

Year2021
JournalJournal of Infection
Journal citation82 (5), pp. 170-177
PublisherElsevier
ISSN1532-2742
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jinf.2021.03.010
Web address (URL)http://europepmc.org/abstract/med/33753152
Publication dates
Online20 Mar 2021
Publication process dates
Accepted15 Mar 2023
Deposited17 May 2023
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