Strain‐Promoted Cycloadditions in Lipid Bilayers Triggered by Liposome Fusion

Journal article


Jumeaux, C., Spicer, C.D., Charchar, P., Howes, P., Holme, M.N., Ma, L., Rose, N.C., Nabarro, J., Fascione, M.A., Rashid, M.H., Yarovsky, I. and Stevens, M.M. (2024). Strain‐Promoted Cycloadditions in Lipid Bilayers Triggered by Liposome Fusion. Angewandte Chemie International Edition. p. e202314786. https://doi.org/10.1002/anie.202314786
AuthorsJumeaux, C., Spicer, C.D., Charchar, P., Howes, P., Holme, M.N., Ma, L., Rose, N.C., Nabarro, J., Fascione, M.A., Rashid, M.H., Yarovsky, I. and Stevens, M.M.
Abstract

Due to the variety of roles served by the cell membrane, its composition and structure are complex, making it difficult to study. Bioorthogonal reactions, such as the strain promoted azide-alkyne cycloaddition (SPAAC), are powerful tools for exploring the function of biomolecules in their native environment but have been largely unexplored within the context of lipid bilayers. Here, we developed a new approach to study the SPAAC reaction in liposomal membranes using azide- and strained alkyne-functionalized Förster resonance energy transfer (FRET) dye pairs. This study represents the first characterization of the SPAAC reaction between diffusing molecules inside liposomal membranes. Potential applications of this work include in situ bioorthogonal labeling of membrane proteins, improved understanding of membrane dynamics and fluidity, and the generation of new probes for biosensing assays.

Year2024
JournalAngewandte Chemie International Edition
Journal citationp. e202314786
PublisherWiley
ISSN1521-3773
Digital Object Identifier (DOI)https://doi.org/10.1002/anie.202314786
Web address (URL)https://doi.org/10.1002/anie.202314786
Publication dates
Online04 Mar 2024
Publication process dates
Deposited15 Mar 2024
Publisher's version
License
File Access Level
Open
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