Downregulation of IGF-1 receptor occurs after hepatic linage commitment during hepatocyte differentiation from human embryonic stem cells

Journal article

Waraky, A, Aleem, E and Larsson, O (2016). Downregulation of IGF-1 receptor occurs after hepatic linage commitment during hepatocyte differentiation from human embryonic stem cells. Biochemical and Biophysical Research Communications. 478 (4), pp. 1575-1581. https://doi.org/10.1016/j.bbrc.2016.08.157
AuthorsWaraky, A, Aleem, E and Larsson, O
Abstract

The insulin-like growth factor 1 receptor (IGF-1R) has been suggested to be involved in hepatocyte differentiation. Human hepatocyte cancer cells and stem cells are known to express IGF-1R whereas normal hepatocytes do not. In the present study we optimized a differentiation protocol and verified the different stages by established markers. The expression levels of IGF-1R and major downstream signaling proteins during differentiation from human embryonic stem cells (hESC) to mature hepatocytes were investigated. We could only demonstrate a minor decrease in IGF-1R expression during endodermal differentiation compared to hESC, but declined substantially (>50%) after hepatic lineage commitment during the hepatocyte specification and maturation stages. This downregulation was paralleled by an upregulation of ERK 1/2, AKT and insulin substrate-1. Neither inhibition nor activation of IGF-1R had any essential effect on endoderm differentiation of human embryonic stem cells. Therefore, our data suggest that IGF-1R downregulation may have a regulatory impact after initiation of hepatic lineage commitment.

Year2016
JournalBiochemical and Biophysical Research Communications
Journal citation478 (4), pp. 1575-1581
PublisherElsevier
ISSN0006-291X
Digital Object Identifier (DOI)https://doi.org/10.1016/j.bbrc.2016.08.157
Publication dates
Print31 Aug 2016
Publication process dates
Deposited12 Dec 2018
Accepted27 Aug 2016
Publisher's version
License
File Access Level
Open
Permalink -

https://openresearch.lsbu.ac.uk/item/87290

Download files

Publisher's version
2017%20POETIC%20Summer%20Meeting%20Agenda_041417.pdf
License: CC BY-NC-ND 4.0
File access level: Open

  • 114
    total views
  • 170
    total downloads
  • 2
    views this month
  • 0
    downloads this month

Export as

Related outputs

Identifying lifestyle factors associated to co-morbidity of obesity and psychiatric disorders, a pilot study
Gaskell, C., Sarada, P., Aleem, E. and Bendriss, D.G. (2023). Identifying lifestyle factors associated to co-morbidity of obesity and psychiatric disorders, a pilot study. Frontiers in Public Health. 11, p. 1132994. https://doi.org/10.3389/fpubh.2023.1132994
Nanoparticle mediated cancer cell therapy: Basic science to clinical applications
Verma, J., Warsame, C., Seenivasagam, R.K., Kumar, N., Abdel Aleem, E. and Goel, S. (2023). Nanoparticle mediated cancer cell therapy: Basic science to clinical applications. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-023-10086-2
Exopolysaccharide-peptide complex from oyster mushroom (Pleurotus ostreatus) protects against hepatotoxicity in rats
Abdel-Monem, NM, El-Saadani, MA, Daba, AS, Saleh, SR and Aleem, E (2020). Exopolysaccharide-peptide complex from oyster mushroom (Pleurotus ostreatus) protects against hepatotoxicity in rats. Biochemistry and Biophysics Reports. 24, pp. 100852-100852. https://doi.org/10.1016/j.bbrep.2020.100852
Clinical resistance associated with a novel MAP2K1 mutation in a patient with Langerhans cell histiocytosis
Azorsa, DO, Lee, DW, Wai, DH, Bista, R, Patel, AR, Aleem, E, Henry, MM and Arceci, RJ (2018). Clinical resistance associated with a novel MAP2K1 mutation in a patient with Langerhans cell histiocytosis. Pediatric Blood and Cancer. 65 (9), pp. e27237-e27237. https://doi.org/10.1002/pbc.27237
Disulfiram overcomes bortezomib and cytarabine resistance in Down-syndrome-associated acute myeloid leukemia cells
Bista, R, Lee, DW, Pepper, OB, Azorsa, DO, Arceci, RJ and Aleem, E (2017). Disulfiram overcomes bortezomib and cytarabine resistance in Down-syndrome-associated acute myeloid leukemia cells. Journal of Experimental & Clinical Cancer Research. 36 (22). https://doi.org/10.1186/s13046-017-0493-5
Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage
Waraky, A, Lin, Y, Warsito, D, Haglund, F, Aleem, E and Larsson, O (2017). Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. Journal of Biological Chemistry. 292 (44), pp. 18227-18239. https://doi.org/10.1074/jbc.M117.781492
Targeting cell cycle regulators in hematologic malignancies
Aleem, E. and Arceci, R.J. (2015). Targeting cell cycle regulators in hematologic malignancies. Frontiers in Cell and Developmental Biology. 3 (APR), pp. 16-. https://doi.org/10.3389/fcell.2015.00016
Picropodophyllin causes mitotic arrest and catastrophe by depolymerizing microtubules via Insulin-like growth factor-1 receptor-independent mechanism
Waraky, A., Akopyan, K., Parrow, V., Strömberg, T., Axelson, M., Abrahmsén, L., Lindqvist, A., Larsson, O. and Aleem, E. (2014). Picropodophyllin causes mitotic arrest and catastrophe by depolymerizing microtubules via Insulin-like growth factor-1 receptor-independent mechanism. Oncotarget. 5 (18), pp. 8379-8392. https://doi.org/10.18632/oncotarget.2292