Dynamic Changes of Inhibitory Killer-Immunoglobulin-Like Receptors on NK Cells after Allogeneic Hematopoietic Stem Cell Transplantation: An Initial Study

Journal article


Dekojová T., Houdová, L., Fatka, J., Pitule, P., Ostašov, P., Gmucová, H., Lysák, D., Jindra, P., Holubová, M. and Caputo Galarce, V. (2020). Dynamic Changes of Inhibitory Killer-Immunoglobulin-Like Receptors on NK Cells after Allogeneic Hematopoietic Stem Cell Transplantation: An Initial Study. Journal of Clinical Medicine. 9 (11), p. 3502. https://doi.org/10.3390/jcm9113502
AuthorsDekojová T., Houdová, L., Fatka, J., Pitule, P., Ostašov, P., Gmucová, H., Lysák, D., Jindra, P., Holubová, M. and Caputo Galarce, V.
Abstract

Killer-immunoglobulin-like receptors (KIRs) are critical natural killer (NK) cell regulators. The expression of KIRs is a dynamic process influenced by many factors. Their ligands—HLA(Human Leukocyte Antigen) class I molecules—are expressed on all nucleated cells that keep NK cells under control. In hematopoietic stem cell transplantation (HSCT), NK cells play an essential role in relapse protection. In the presented pilot study, we characterized the dynamic expression of inhibitory KIRS (iKIRs), which protect cells against untoward lysis, in donors and patients during the first three months after HSCT using flow cytometry. The expression of all iKIRs was highly variable and sometimes correlated with patients’ clinical presentation and therapy regiment. Cyclophosphamide (Cy) in the graft-versus-host disease (GvHD) prevention protocol downregulated KIR2DL1 to just 25% of the original donor value, and the FEAM (Fludarabine + Etoposid + Ara-C + Melphalan) conditioning protocol reduced KIR2DL3. In lymphoid neoplasms, there was a slightly increased KIR2DL3 expression compared to myeloid malignancies. Additionally, we showed that the ex vivo activation of NK cells did not alter the level of iKIRs. Our study shows the influence of pre- and post-transplantation protocols on iKIR expression on the surface of NK cells and the importance of monitoring their cell surface

Keywords KIRs; NK cells; HSCT
Year2020
JournalJournal of Clinical Medicine
Journal citation9 (11), p. 3502
PublisherMDPI
ISSN2077-0383
Digital Object Identifier (DOI)https://doi.org/10.3390/jcm9113502
Publication dates
Online29 Oct 2020
Publication process dates
Accepted28 Oct 2020
Deposited17 Jun 2021
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Open
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